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KMID : 0903520090520050466
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 5 p.466 ~ p.471
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Factors Involved in Masspropagation of Ginseng (Panax ginseng C. A. Meyer) using Bioreactor System
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Lee Jae-Geun
Seong Eun-Soo
Goh Eun-Jeong
Kim Na-Young
Yu Chang-Yeon
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Abstract
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We investigated suitability of media and the types and concentrations of growth regulators necessary for establishing bioreactor cultures of mountain-grown ginseng. Effects of cytokinin and auxin on root growth differed with culture and accession. Low levels of cytokinin and high levels of auxin proved to be superior root-growth regulators. SH (Sheck & Hildebrant) culture medium produced better growth than blends of MS (Murashige & Skoog) and WPM (Wood plant medium) media. The best suspension culture for induction of roots was SH medium supplemented with 4 mg/L indole-3-butyric acid (IBA). Root growth under 1700 lux illuminance was reduced by as much as 10% relative to that under 3500 lux.
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KEYWORD
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auxin, bioreactor, cytokinin, ginseng, light, sucrose
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